Hogeschool Gent
Geraard de Duivelstraat 5 - 9000 Gent
Tel: 09 243 33 33 - E-mail: info@hogent.be
Website: www.hogent.be
Medical bacteriology 230601/4115/1819/1/68
Study guide

Medical bacteriology 2

Academic year 2018-19
Is found in:
  • English programme for Health, Education and Welfare, programme stage 3
This is a single course unit.
Study load: 5 credits
Weight: 5,00
Total study time: 135,00 hours
Possible deadlines for learning account: 01.12.2018 ()

Organisation of education

Teaching Activities, Learning Activities, Assessment Activities
Lecture20,00 hours
Self-study75,00 hours
Seminar40,00 hours
This course unit is marked out of 20 (rounded to an integer).
Re-sit exam: is possible.
It is not possible to enrol in this course unit under
  • exam contract (to obtain a credit).
  • exam contract (to obtain a degree).
Co-ordinator: De Pauw Isabel
Other teaching staff: Bens Ann, Van Mechelen Els
Language course: No
Languages: English
Scheduled for: Semester 1


We can motivate why we included both partial course units in the study program.
The aim of this unit is the acquisition of a good insight and active knowledge concerning strategy and manipulations on clinical specimens in a routine microbiology lab, namely the microscopic examination, the biochemical and molecular identification and typing of human bacterial pathogens, the implementation of a quality control system in a medical routine microbiological lab and susceptibility testing. Students learn about the pathogen gram-positive bacteria (cocci and rods), Mycobacteria, Spirochaetales, Chlamydia and Mycoplasmata. The pre analytical phase is treated with an overview of the different clinical samples. Students learn to distinguish pathogen from commensal flora in the different clinical specimens. Students are trained to read, comprehend and apply scientific literature concerning medical bacteriology.

This unit is included in the curriculum in order to perform accurately sterile manipulations in medical routine microbiological labs hospital and private medical labs), with respect for biosafety. Students are trained to carry out microscopic examination, to work with basic equipment in a microbiological lab, to culture and isolate bacterial colonies from different clinical specimens (urine, faeces, sputum), to carry out molecular and biochemical identifications, to perform susceptibility testing, to report accurately the experiments and to interpret laboratory results. The student will also acquire basic skills in applications of bio-informatics, molecular and biochemical diagnostic analysis methods and strategies, the use of biosafety cabinets and sterile culturing of bacteria (Enterobacteriaceae, non-fermenting gram negative rods, gram positive cocci). Students are trained to read, comprehend and apply scientific literature concerning medical bacteriology


Pre analytical phase : clinical specimen types, specimen collection, identification, transport of specimens, quality control
Human bacterial pathogens : gram negative cocci, grampositive cocci,aerobic and anaerobic grampoistive rods, anaerobic gramnegative rods, Spirochaetales, Mycobacterium, Mycoplasmata, Chlamydia
Strategies for identifying bacterial pathogens in different types of clinical specimens
Practicum 1 : MRSA - screening
Practicum 2 : Identification of a bacterial pathogen in urine
Practicum 3 : Identification of a bacterial pathogen in faeces
Practicum 4 : quantification of Pseudomonas aeruginosa in sputum of cystic fibrosis patients, a comparison of qPCR and colony counting on cetrimide agar plates
Practicum 5 : Identification of a bacterial pathogen in urine and faeces
Practicum 6 : sterile cultering of eukaryotic cell lines

Order of enrolment

There are no prerequisites for this course.


The agreements on sequentiality who apply for this course in the education programme are found in the teaching and examination regulations (FOER).

Final objectives

Domain-specific Learning Outcome 4
Professional bachelor students of Bio-Medical Laboratory Technology are actively involved in an organised quality system in the laboratory.
Domain-specific Learning Outcome 6
Professional bachelor students of Bio-Medical Laboratory Technology work in a safe and environmentally-aware manner in accordance with statutory provisions within a lab setting, both in a chemistry lab and in a class II biosafety lab.
Domain-specific Learning Outcome 8
Professional bachelor students of Bio-Medical Laboratory Technology submit clear and correct reports on research data, analysis results, projects and processing of scientific literature, using the appropriate biomedical specialist terminology in languages other than Dutch.
Domain-specific Learning Outcome 9
Professional bachelor students of Bio-Medical Laboratory Technology communicate adequately with the target groups. They collaborate in a team constructively and respectfully within an intra- and inter-professional context.
Domain-specific Learning Outcome 10
Professional bachelor students of Bio-Medical Laboratory Technology make an active contribution to the practical completion of biomedical, scientific research on the basis of scientific sources and current developments.

Profession-specific Learning Outcome M 12 Medical laboratory technology
Professional bachelor students of Pharmaceutical and Biological Laboratory Technology apply the appropriate methods and technologies of the medical-diagnostic field in a scientifically substantiated manner and in varying contexts…

Professionn-specific Learning Outcome M12.1
… within the area of microbiology
Profession-specific Learning Outcome M12.4
… within the area of molecular diagnostics.

Organisation of education

Interactive lectures

Seminars and hands-on workshops in which the teacher guides a small group of students

Study guidance

Difficulties in understanding and acquiring the subject matter and/or acquiring the required skills are reported by the student or noticed by the teacher and corrected by individual supervision of students during practical sessions or if necessary through a classroom remediation.
Peertutoring is used to remedy difficulties in acquiring the course material.
The teacher traces gaps through continuous evaluation (assessing reports and interim tests and observation of practical courses).


Examination methods in case of periodic evaluation during the first examination period
Written exam (70% of the marks)
Permanent evaluation (PE) or non-periodic evaluation (30% of the marks)
Labnotes, tests, assignments, presentation

Presence during lessons and sessions is necessary.
Cf. EER HoGhent (2018- 2019) and EER -Faculty Education, Health and Social Work (2018- 2019)

Examination methods in case of periodic evaluation during the second examination period
An analogous written exam is organized during the resist (70% of the marks)
A second chance evaluation for the non-periodic evaluation during the second evaluation period is excluded. The results of the non-periodic evaluation of the first evaluation period (30% of the marks) are retained.
Cf. EER HoGhent (2018- 2019) and EER -Faculty Education, Health and Social Work (2018- 2019)

Study costs

The cost of the books mentioned under the heading 'Study Materials: Required' is estimated at around 25 euro. The books are used in the course units Medical Bacteriology 1 and 2.
Costs for printing of course notes and additional notes and powerpoint presentations is estimated at 15 €.

Other study costs are estimated at about 25 euros: a mandatory fireproof labcoat with Biosafety label (one time purchase for all practical biosafety risks).

Key words

Gram positive bacteria
Clinical samples
Human infections
Pre analytical phase
Identification strategy