Hogeschool Gent
Geraard de Duivelstraat 5 - 9000 Gent
Tel: 09 243 33 33 - E-mail: info@hogent.be
Website: www.hogent.be
Biotechnology30599/3978/1718/1/46
Study guide

Biotechnology

30599/3978/1718/1/46
Academic year 2017-18
Is found in:
  • English programme for Health, Education and Welfare, programme stage 3
This is a single course unit.
Study load: 9 credits
Weight: 9,00
Total study time: 243,00 hours
Possible deadlines for learning account: 01.12.2017 ()

Organisation of education

Teaching Activities, Learning Activities, Assessment Activities
Lecture35,00 hours
Self-study127,00 hours
Seminar56,00 hours
Supervised independent work25,00 hours
This course unit is marked out of 20 (rounded to an integer).
Re-sit exam: is possible.
It is not possible to enrol in this course unit under
  • exam contract (to obtain a credit).
  • exam contract (to obtain a degree).
Co-ordinator: De Pauw Isabel
Other teaching staff: Bens Ann, Van Mechelen Els
Language course: No
Languages: English
Scheduled for: Semester 1

Objectives

We can motivate why we included this course unit in the study program.
Theory
This unit is included in the curriculum in order to acquire basic knowledge in biotechnology. The aim of this partial course unit is the acquisition of a good insight and active knowledge on DNA-technology. The student will also have knowledge of the possibilities and applications of bio-informatics, molecular diagnostic analysis methods, different cloning strategies, the production of recombinant proteins and culturing of cells and cell lines. This knowledge will lead the student to understand and perform accurately the different biotechnological research strategies in medical routine and biomedical research labs (university and pharmaceutical industry).
Students are trained to read, comprehend and apply scientific literature concerning biotechnology.
Practicum
This unit is included in the curriculum in order to perform accurately biotechnological manipulations in medical routine and biomedical research labs (university and pharmaceutical industry), with respect for biosafety. Students are trained to carry out the necessary calculations, to work with basic equipment in a biotechnology lab, to report accurately the experiments in a hand written lab notebook and to interpret laboratory results. The student will also acquire basic skills in applications of bio-informatics, molecular diagnostic analysis methods and cloning strategies, the production of recombinant proteins and culturing of cells and cell lines.
Students are trained to read, comprehend and apply scientific literature concerning biotechnology.

Contents

Theory
Chapter 1 : basic tools in recombinant DNA-technology
Chapter 2 : molecular diagnostic technology in genetics, hematology and forensic analysis
Chapter 3 : cloning of DNA
Chapter 4 : Isolation and purification of recombinant proteins
Chapter 5 : Biologicals
Visit to biotechnological companies, theme : development and production of biologicals and quality control systems
Practicum
Unit 1 : Hands on practicum software for cloning and software for primerdesign
Unit 2 : Sterile culture of eukaryotic cell lines
Unit 3 : Introduction to practical biotechnology laboratory work
Unit 4 : Cloning of a human DNA sequence
Unit 5 : Isolation, purification and quality control of a recombinant protein

Order of enrolment

There are no prerequisites for this course.

Prerequisites

The agreements on sequentiality who apply for this course in the education programme are found in the teaching and examination regulations (FOER).

Final objectives

Domain-specific Learning Outcome 2
Professional bachelor students of Bio-Medical Laboratory Technology carry out biomedical laboratory activities in a substantiated manner autonomously, reliably and critically. They establish problems, report them and contribute to an acceptable solution.
Domain-specific Learning Outcome 5
Professional bachelor students of Bio-Medical Laboratory Technology apply the ethical principles of the biomedical field.
Domain-specific Learning Outcome 6
Professional bachelor students of Bio-Medical Laboratory Technology work in a safe and environmentally-aware manner in accordance with statutory provisions within a lab setting, both in a chemistry lab and in a class II biosafety lab.
Domain-specific Learning Outcome 8
Professional bachelor students of Bio-Medical Laboratory Technology submit clear and correct reports on research data, analysis results, projects and processing of scientific literature, using the appropriate biomedical specialist terminology in languages other than Dutch.
Domain-specific Learning Outcome 9
Professional bachelor students of Bio-Medical Laboratory Technology communicate adequately with the target groups. They collaborate in a team constructively and respectfully within an intra- and inter-professional context.
Domain-specific Learning Outcome 10
Professional bachelor students of Bio-Medical Laboratory Technology make an active contribution to the practical completion of biomedical, scientific research on the basis of scientific sources and current developments.

Domain-specific Learning Outcome F 12 Pharmaceutical and biological laboratory technology
Professional bachelor students of Pharmaceutical and Biological Laboratory Technology apply the appropriate methods and technologies of the medical-diagnostic field in a scientifically substantiated manner and in varying contexts…

Domain-specific Learning Outcome F12.1
… within biological research. In doing so, they apply bioinformatics applications as and when required
Domain-specific Learning Outcome F12.2
... within the area of pharmacology and toxicology

Organisation of education

Theory
Interactive lectures
Video
Company visit

Practicum
Seminars and hands-on workshops in which the teacher guides a small group of students
Peertutoring
Video

Study guidance

Difficulties in understanding and acquiring the subject matter and/or acquiring the required skills are reported by the student or noticed by the teacher and corrected by individual supervision of students during practical sessions or if necessary through a classroom remediation.
Peertutoring is used to remedy difficulties in acquiring the course material.
The teacher traces gaps through continuous evaluation (assessing reports and interim tests and observation of practical courses).

Evaluation

Evaluation
Examination methods in case of periodic evaluation during the first examination period
Theory (70 % of the marks)
Written exam
Paper
Practicum(30% of the marks)
Permanent or non-periodic evaluation
Labnotes, tests, assignments
Presence during lessons and sessions is necessary.
Cf. EER HoGhent (2017- 2018) and EER -Faculty Education, Health and Social Work (2017- 2018)
Examination methods in case of periodic evaluation during the second examination period

An analogous written exam is organized during the resist.
A second chance evaluation for the permanent or non-periodic evaluation during the second evaluation period is excluded. The results of the non-periodic evaluation of the first evaluation period are retained.
Cf. EER HoGhent (2017- 2018) and EER -Faculty Education, Health and Social Work (2017- 2018)

Study costs

Costs for printing of course notes and additional notes and powerpoint presentations is estimated at 25 €.
Other study costs are estimated at about 55 euros.
Other study costs are estimated at about 25 euro: a mandatory fireproof labcoat with Biosafety label (one time purchase for all practical biosafety risks).

Key words

Theory
Recombinant gene-expression, recombinant proteins, genetic engineering, biologicals, GMP, cell culture, gene therapy

Practicum
qPCR, cell culture, primerdesign, cloning, western blotting, transformation